A royal flush is the strongest hand (or card combination) in texas holdem. Fluttershy would've definitely won the round hadn't she 'folded' her royal flush (to 'fold' means to cease your own participation in a round). She is very shy and tactful, so she didn't want to hurt anypony's feelings by winning and decided to fold her top-rank combo.
with me: me: -sees royal flush in my hand- twi: well? me: all in -takes all bits (100) and puts them on table- all other mane 6: ?! your gonna loose!! me: i know what i'm doing AJ: fold pinkie: 2 pair RD: full house twi: three of a kind flutter: four of a kind me: royal flush mane 6: 0.0
That could work too. Right now, I was also thinking about the Game Corner theme from Pokémon Gold/Silver/Crystal or Pokémon HeartGold/SoulSilver (whichever one you want), or the Casino Night Zone theme from Sonic The Hedgehog 2.